bla
Menschliche Genomvariation
Genetic and physical maps
- Definition
- Einheit
997
Menschliche Genomvariation
Linkage disequilibrium
- Prinzip
996
Menschliche Genomvariation
Linkage disequilibrium; Quanitifikation
- D
--> Prinzip, Berechnung
995
Menschliche Genomvariation
Linkage disequilibrium; Quanitifikation; D
- Nachteile von D
- -> Angabe von L ohne diese Nachtteile
994
Menschliche Genomvariation
Linkage disequilibrium
- was wird dadurch ermöglicht?
993
Menschliche Genomvariation
Haplotype blocks
- Definition
992
Menschliche Genomvariation
Triangle plot
- Zweck
991
Menschliche Genomvariation
Haplotype blocks
- dependence on population hisory
990
Menschliche Genomvariation
Linkage analysis
- Prinzip
989
Menschliche Genomvariation
Linkage analysis
- Einheit
988
Menschliche Genomvariation
Linkage analysis; Example: May-Hegglin Anomaly
- was ist das für eine Krankheit?
987
Menschliche Genomvariation
Linkage analysis; Example: May-Hegglin Anomaly
- identifying the Gene
986
Menschliche Genomvariation
Assosciation studies
- Prinzip
985
Menschliche Genomvariation
Assosciation studies
- Numbers of cases and controls required for genomewide association studies
984
Menschliche Genomvariation
Assosciation studies
- A whole genome disease association study
for type 2 diabetes
983
Menschliche Genomvariation
Cancer genomics
- Goals
982
Menschliche Genomvariation
Cancer genomics; Gene centered approaches
-Prinzip
981
Menschliche Genomvariation
Cancer genomics; Whole genome projects
-Prinzip
- driver mutations vs passenger mutations
980
Menschliche Genomvariation
Cancer genomics
- using of expression analysis
979
Platzahler
...
Einführung
Genetics vs genomic vs functional genomics
781
Einführung
Funtional genomics; gene Expression anaysis
492
Einführung
Pharmacogenomics
- definition
-> Problem
493
Technolgie
Gelelektrophorese
-> Prinzip
494
Technolgie
DNA Sequenzierung; Sanger Methode
- Prinzip
495
Technolgie
DNA Sequenzierung;Capillary electrohoresis
496
Technolgie
DNA Sequenzierung; Pyrosequenzierung
- Prinzip
497
Technolgie
DNA Sequenzierung; Sequencing by Synthesis
- Prinzip
Sequencing by synthesis is also commonly referred to as «Illumina sequencing», for the name of the company that has developed the technology. It is one of the most popular high throughput sequencing technologies, because it produces massive amounts of accurate sequencing data.
It is performed on flow cells that are similar to microscope slides, but where each cell contains eight sealed reaction compartments (lanes) in which the sequencing reactions take place. Each lane can be used to sequence a separate sample of DNA, e.g., a separate genome.
Each lane is covered by a dense lawn of short, single stranded pieces of DNA (oligonucleotides) whose purpose is to hybridize to the DNA to be sequenced, to anchor this DNA to the flow cell, and to serve as primers for DNA polymerase. There are two different types of such oligonucleotides, each with a specific nucleotide sequence.
The process is also described in the first 2:30 minutes of the following video (the remainder of the video covers aspects of SBS that are more complex than those we cover in this class): https://www.youtube.com/watch?v=HMyCqWhwB8E
Technolgie
DNA Sequenzierung; Future tehnologies
-> Ansatz
499
Technolgie
DNA Sequenzierung; Geschwindigeit
-> im Lufe der Jahre
500
Technolgie
Whole-genome shotgun sequencing
- Prinzip
- Problem
710
Technolgie
Whole-genome shotgun sequencing
-> Finishing
- Genauigkeit
711
Technolgie
Whole-genome shotgun sequencing
-> Sequencing coverage
712
Technolgie
Whole-genome shotgun sequencing
-> Sequencing annotation
- ziel
713
Technolgie
Sizes of genomes and numbers of genes ca. 3x109 base pairs
-> humans vs E coli
714
Technolgie
Genomewide expression analysis
- Ziel / prinzip
715
Technolgie
Microarrays
- was kann man damit messen
- Vorteil
- (grobes) prinzip
716
Technolgie
Hybridization
- Prinzip /Definition
717
Technolgie
Northern Blot
- nachteil gegenüber Microarrays
718
Technolgie
Basics of microarrays
- Prinzip
- cDNA
719
-
- 1 / 90
-
Lernkarten
Einführung
Genetics vs genomic vs functional genomics
781
Einführung
Funtional genomics; gene Expression anaysis
492
Einführung
Pharmacogenomics
- definition
-> Problem
493
Technolgie
Gelelektrophorese
-> Prinzip
494
Technolgie
DNA Sequenzierung; Sanger Methode
- Prinzip
495
Technolgie
DNA Sequenzierung;Capillary electrohoresis
496
Technolgie
DNA Sequenzierung; Pyrosequenzierung
- Prinzip
497
Technolgie
DNA Sequenzierung; Sequencing by Synthesis
- Prinzip
Sequencing by synthesis is also commonly referred to as «Illumina sequencing», for the name of the company that has developed the technology. It is one of the most popular high throughput sequencing technologies, because it produces massive amounts of accurate sequencing data.
It is performed on flow cells that are similar to microscope slides, but where each cell contains eight sealed reaction compartments (lanes) in which the sequencing reactions take place. Each lane can be used to sequence a separate sample of DNA, e.g., a separate genome.
Each lane is covered by a dense lawn of short, single stranded pieces of DNA (oligonucleotides) whose purpose is to hybridize to the DNA to be sequenced, to anchor this DNA to the flow cell, and to serve as primers for DNA polymerase. There are two different types of such oligonucleotides, each with a specific nucleotide sequence.
The process is also described in the first 2:30 minutes of the following video (the remainder of the video covers aspects of SBS that are more complex than those we cover in this class): https://www.youtube.com/watch?v=HMyCqWhwB8E
Technolgie
DNA Sequenzierung; Future tehnologies
-> Ansatz
499
Technolgie
DNA Sequenzierung; Geschwindigeit
-> im Lufe der Jahre
500
Technolgie
Whole-genome shotgun sequencing
- Prinzip
- Problem
710
Technolgie
Whole-genome shotgun sequencing
-> Finishing
- Genauigkeit
711
Technolgie
Whole-genome shotgun sequencing
-> Sequencing coverage
712
Technolgie
Whole-genome shotgun sequencing
-> Sequencing annotation
- ziel
713
Technolgie
Sizes of genomes and numbers of genes ca. 3x109 base pairs
-> humans vs E coli
714
Technolgie
Genomewide expression analysis
- Ziel / prinzip
715
Technolgie
Microarrays
- was kann man damit messen
- Vorteil
- (grobes) prinzip
716
Technolgie
Hybridization
- Prinzip /Definition
717
Technolgie
Northern Blot
- nachteil gegenüber Microarrays
718
Technolgie
Basics of microarrays
- Prinzip
- cDNA
719
Technolgie
microarrays
- Target labeling: fluorescent cDNA
-> prinzip
720
Technolgie
microarrays; spotted Microarray
- Prinzip
721
Technolgie
microarrays; Synthesis on the Chip
- Prinzip
722
Technolgie
microarrays; Microrray Hybridization
- Prinzip
723
Technolgie
microarrays; Microrray Hybridization
- 2 methoden
724
Technolgie
microarrays;Labels
- Exampels
725
Technolgie
microarrays; scanning
- Prinzip
726
Technolgie
microarrays; Microarrays and cancer
- Vorteil
727
Technolgie
microarrays; Profiling Tumors
- Prinzip
728
Technolgie
microarrays; Microarrays in the prognosis of metastasis
- Prinzip
729
Technolgie
microarrays; Analysis of microarray results
- Replicates
730
Technolgie
RNA- Seq
- Prinzip
731
Technolgie
Comparing microarrays and RNA-seq
Tabelle
732
Technologie
Proteomics
- Definition
- Ziel
886
Technologie
Proteomics
- Problem
-> verglichen mit genomics
887
Technologie
Proteomics
Diversity of function in splice variants
- Beispiel Calcitonin gen
888
Technologie
Proteomics
Expected practical applications of proteomics
889
Technologie
Proteomics
Technologies for proteomics
- was für technologien werden angewendet?
890
Technologie
Proteomics
Mass spectrometry
- was misst man damit?
- Welche 4 komponenten braucht jedes massenspektrometer?
891
Technologie
Proteomics
MALDI
- Prinzip
892
