M1

Genomics it the science of understanding of cellular and organism genomes (size, organisation, composition) (complete DNA sequence). It uses gemone sequencing and analysis tools. Coding and noncoding sequences are detected.

23andMe = personal genome test

human samples are tested using targeted sequencing of specific genomic locations based on published research.

Transcriptomics aims to explain genexpression  (RNA-molecules, coding molecules), which genes are actually expressed.

reduced sequencing effort. only coding sequneces are detected (but provides no information about gene expression, just potentially available gene functions)

aims to detect the amount of proteins expressed.

aims to describe the phenotype of a cell "function", quantitative summary of all metabolites under certain conditions.

:-( high technical, bioinformatic and financial efford, large amount of data, high error rate, needs many different and multiple reads

1) random fragmentation of DNA (mechanistical shearing, enzymatic restriciton)

2) sequencing random fragments (overlap phase)

3)bioinformatic assembly of short fragmentes to build genome consensus