Vortrag
C. Elegans
C. Elegans
Set of flashcards Details
| Flashcards | 32 |
|---|---|
| Language | English |
| Category | Biology |
| Level | University |
| Created / Updated | 08.07.2020 / 08.07.2020 |
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C. elegans exists primarily...however...
as a self-fertilizing hermaphrodite...males which arise from meiotic non-disjunction also occur with a frequency of about 0,2 %
In the picture you can see the two sexes in the adult stage. Hermaphrodites
have a wider girth and a tapered tail, while males have a slimmer girth and a fan-shaped tail
Einleitung self- und cross-fertilization
from a genetic point of view both self- and cross-fertilization have their advantages
Einleitung: differentiate self- and cross-progeny
There is a simple method to clearly differentiate between self- and cross-progeny based on the visible phenotype. In this case there is a different in locomotion through a mutation called roller
As you can see the animal twist their body like a corkscrew
and as a result often remain in the same region moving in circular pattern
the left site shows how the germ cells
recombined after a hermaphrodite fertilizes its oozytes with its own sperm
But when the oocyte is fertilized by the sperm of a male,
all progeny did not have the roller phenotype because the copy of the wildtype roller gene from the male always complement the mutation
einleitung methoden
now i would like to introduce you to some common used methods
worm sorters
to facilitate work with large numbers of worms, worm sorters can be used. It can be used to quickly select large quantities of individual worms with desired characteristics
einleitung RNA interference
RNA interference is used to silence genes in order to determine its function
IN 1998 RNA interference was first discovered and characterized in c. elegans. the method used
double stranded RNA to reduce gene activity
Einleitung Research
So let´s talk about the research in C. elegans
Einleitung million mutation project
One of the most important studies in C. elegans was the million mutation project
Einleitung research fields
C. elegans is not only an exellent model for the nervous system. It also used in many other research fields. In the following I go through the tissues in which research is carried out with c. elegans and give you for each two examples
Einleitung limits/disadvantages
No model organism can be used to answer every research question, and working with c. elegans has some limitations and disadvantages
Title paper
A transparent window into biology: a primer on Caenorhabditis Elegans
Einleitung topics
My presentation is divided into six parts
Topics: advantages as model organism
I will start to introduce you to the advantages of c. Elegans as a model organism
Topics: genetics for self- and cross-fertilization
then i will show you how the ability of self- and cross-fertilization facilitate genetic work
Topics methods and research
Afterwards I will tell you something about common used methods and give you an insight into Research fields
Topics: limits/disadvantages
I will also talk about limits and disadvantages
Topics: summary
And in the end i will summarize the most important points
small size
C. elegans is a small organism with a length of only 1 mm. So a single petri dish can hold about ten thousand worms
short life cycle and large brood size
the life cycle is very short. The generation time is about 3 days with more than 300 progeny at each generation. This is an important advantage for statistical analysis
short lifespan
with a lifespan of 2-3 weeks, C. elegans is also suitable for age research
transparency
its transparent body allows to easily follow cell fate or expression of fluorescently tagged proteins of interest in the living animal
ease of isolation, cultivation, and maintance
isolation, cultivation and maintance are very simple
temperature-dependend growth
growth at different temperatures makes it possible to control the rate of development and can be used for temperature-sensitive mutants
ease of genetic manipulation
a major reason why sidney brenner choosed C. elegans is the ease of genetic manipulation including forward and reverse genetic
ability to freeze and recover
the ability to freeze and recover makes it possible to preserve mutant strains
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