Molecular Biotechnology&Genomics
Information to learn off by heart
Information to learn off by heart
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Kartei Details
| Karten | 7 |
|---|---|
| Sprache | Deutsch |
| Kategorie | Biologie |
| Stufe | Universität |
| Erstellt / Aktualisiert | 20.01.2020 / 23.01.2020 |
| Lizenzierung | Keine Angabe |
| Weblink |
https://card2brain.ch/box/20200120_molecular_biotechnologygenomics
|
| Einbinden |
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Illumina (Nucleotide detection, Read length, total bases/run, accuracy, cost(euro)/Mb)
- Nucleotide detection: Reversible dye terminators
- Read length: 100-300bp
- total bases/run: 36 Gb/run
- accuracy: 99%
- cost(euro)/Mb: 0.1
- Sequencing by synthesis
Pacific Biosystems
- Nucleotide detection: Phospho-linked fluorescent nucleotides
- Read length: 100-14'000bp
- total bases/run: 100 GB
- accuracy: 85-95%
- cost(euro)/Mb: 1.5
- Sequencing by synthesis
Oxford Nanopore
- Nucleotide detection: Change in membrane potential due to nucleotides
- Read length: 4*103-105
- total bases/run: 2.5-5
- accuracy: 90%
- cost(euro)/Mb: 0.5-1.0
- Direct sequencing
3 Domninat markers + 3 auxotrophic markers
(which ones have marker recycling and how?)
Auxotrophic
- URA3, repairs uracil deficiency: negative selection with 5-FOA (marker recycling)
- LYS2, repaires lysine deficienc: neg. sel with alpha-aminoadipate
- MET15, repairs methionine deficiency: neg. sel with methyl mercury
Dominant markers (no marker recycling):
- KanMX, resistance to G418
- nat, resistance to nourseothricin
- DsdA, resistance to D-serine
Restriction sites:
BsmBi, BsaI
BsmBi: 5' CGTCTC(N) 3'
BsaI: 5' GGTCTC(N) 3'
3 Type specific overhangs
CCCT
AACG
TATG
Stop codons (DNA sequence, 3)
TGA
TAG
TAA
