2013 HSC COURSE
HSC biology, physics, economics, english & four unit mathematics
HSC biology, physics, economics, english & four unit mathematics
Kartei Details
Karten | 417 |
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Sprache | English |
Kategorie | Allgemeinbildung |
Stufe | Mittelschule |
Erstellt / Aktualisiert | 10.04.2013 / 10.09.2024 |
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experiment: pH on enzyme activity
aim: to test the effects of a change in pH on activity of enzyme catalyse
hypothesis: reaction rate will be greatest at pH 7 and lower either side
risk: H202 is corrosive. As such wear gloves and safety glasses
independant: pH
dependant: reaction rate (height of foam)
controlled variables: temperature, substrate and enzyme concentration, time
control: reaction rate should be highest at pH 7
materials: 1 x liver piece 1x 180mL H202 1x pipette 6x buffering solutions (pH 2,4,6,7,8,10), 1x ruler, 1x times, 1x detergent 1x waterbath at 37 degrees celcius 6x testtubes (1 for each pH level)
method: 1. cut up the liver into 0.5 cm square pieces 2. put 10mL h202 and a 0.5 cm square liver piece into seperate testtubes 3. buffer both at pH 2 4. acclimatise both using the waterbath 5. add 3 drops of detergent to the H202 6. combine testtubes and record height 7. repeat 1-6 2 more times 8. repeat 1-7 with other buffering solutions
Temperature on enzyme activity
As temperature increases the collision rate of enzymes and substrate increases however the number of enzymes not denatured decreases
too hot = enzyme collision rate high but enzymes denatured
too cold = enzyme collion rate too low to start reactions
Blood pH
usually pH 7.2
can cope with +- 0.2 changes
beyond this there is a detrimental effect on enzyme efficiency as enzymes denature
Sex Linked inheritance
refers to the expression of an allele that is dependant on the sex of the individual and therefore is directly linked to the sex chromosomes
most sex linked genes are present on the X chromosome are present on the X chromosome and there is no corrosponding allele on the Y chromosome (as smaller - therefore contains less genes) Known as X linked
Few traits are y linked
doesnt follow normal mendellian ratios
Active transport in the kidneys
required energy (ATP) - movement of molecules against a concentration gradient (low concentration -->high)
Reabsorption in the kidneys of ions, glucose, salts, potassium, amino acids
percentages of whole blood
.RBCS - 45%
WBC + Platelets - <1%
PLASMA - 55%
Stages of natural selection
V - in any species there is variation
E - There is an environmental pressure. Organisms with advantagous variations will have a greater change of survival
R - Surviving organism will pass on their genes (in which organisms ith adv variation will be more likely to do so)
A - as the variation becomes predominant in the population it is said to become an adaption
Peritoneal dialysis
Type of dialysis that occurs in the body
Utilises the peritoneal membrane that lines the peritoneum in the abdominal cavity
The peritoneum is filled with dialysis solution (dialysate) through a catheter
The dialysate pulls waster product from the blood through the semi permeable membrane
After 4-6 hours the fluid is drained
Dialysis
The clinical purification of blood
2 types : peritoneal and haemodialysis
experiment: to estimate and draw scale diagrams of WBCs and RBCs
aim: to estimate and draw scale diagrams of RBCs and WBCs using a microscope
hypothesis: RBC ~7-8 micrometres, WBCs ~15 micrometres
risk: microscope is heavy and cause potentially cause damage to feet if dropped. Therefore hold with both hands firmly at the base
materials: 1x light microscope 1x blood smear slide 1x minigrid 1x compass 1x pencil
Method: 1. set up light microscope and place minigrid 2. measure the field of view of the microscope by using the minigrid slide (increasing initially from 10x magnification up to 400x magnification) 3. place the blood smear slide on the microscope and slowly work way up from 10x mag to 400x magnification. 4. using the field of view estimate the size of the RBCs by counting the total number of RBCs in the diametre of the field of view and divide view of view diametre by the number 6. repeat 3 times 7. get average size and record 8. draw scale diagram of RBC using a scale of 1cm = 1 micrometre and mark key features 9.repeat 4-8 with WBCs
Example of enantiostasis
estuarine organisms (osmoconformers) have to experience large changes in salt water concentration throughout the day.
It is too energy consuming to deal as a osmoregulator would so they carry out enantiostasis
Alter internal pH to neutralise effect of different salt water concentrations
e.g. hight tide = high salt concentration therefore they increase their pH
Feedback mechanism
A loop system in which the system responds to perturbation either in the same direction (positive feedback) or in the opposite direction (negative feedback). used in homeostasis as the level of one substance / activity influences the level of another substance or activity
pH scale
logarithmic scale used for measuring the acidity or alkalinity of a substance
pH 7 - neutral
pH < 7 - acidic
pH > 7 basic / alkaline
Australian examples of plant adaptions to waterloss
Banksia - thick waxy cuticle
Cacti - small leaf SA, low number of stomate, extensive roots
Spinifex grass - Low stomata number, sunken stomata, trichomes and rolled leaves
Nitrogenous wastes can be composed of
urea, uric acid, creatinine, ammonia
Digested products include
sugars, vitamins, amino acids
What is cyroprecipitate
The precipitate of thawing frozen plasma between 1 to 6 degrees celcius
Addison's disease
Disease in which the adrenal glands do not produce enough hormones including aldosterone
results:
- too much sodium and H20 and potassium excreted by kidneys affecting blood pressure and heart function
Waste types of Animals
Freshwater fish - ammonia - very toxic - highly soluable
Human, Saltwater fish and mammals - urea - med toxic - med soluable
Birds, insects and reptiles - uric acid - low toxic - low soluability
Mangrove adaptions for saline conditions
1. salt excreted through minute glands in the surface layer of leaves
2. excess salt stored in older leaves before they fall
3. Layer of cells that actively restrict movement of salt into xylem cells
Pedigrees
Shows the inheritance of particular traits through a family tree
used in
1. genetic studies to trace occurance of genetic disease over several genes
2. advise probablility of having an affected child
3. zoo management for breeding
Codominance
Both alleles are independantly and equally expressed in the heterozygote
e.g. roan (stipled red and which) coat colour in cattle
does not follow mendellian ratios
Enzyme concentration on enzyme activity
enzyme activity will increase with enzyme concentration untill a V max i reached - where all substrate is used or connected to active sites
Biogeography
evidence for natural selction
Studies of animal / plant distribution
e.g. ratites - flightless bired e.g. emu, rhea, ostrich, moa all originated from gondwana evolved differently from experiencing different selective pressures
Hybridisation
The offspring of a cross between 2 different species, subspecies or varieties
Hybrids between 2 species are usually infertile as chromosomes dont match up (e.g. donkey and horse)
Hybridisation helps to increase genetic variety
Many hybrids are described as having hybrid vigour - combine best features of parents
e.g of hybrids
groodle = golden retreiver cross poodle
doesnt shed - low allergy (from poodle)
water loving (golden retreiver)
large build (both)
asthetic
High IQs of both - greater trainability
Environment on gene expression
Appearance of an individual is based upon both genotype and environment
e.g. hydranges flower colour is based on soil pH. acidic - blue. alkaline soil - pink. pH has an effect on the availability of other ions in the soil and these ions determine colour change
e.g. Phenylketonuria (PKU) genetic disorder may be controlled by limiting diet
Tension cohesion tension method
Method explaining the movement of water in the xylem tissue
There is:
cohesion between the water molecules (formation of hydrogen bond)
adhesion between the water molecules and the wall of the xylem
transpiration - as water evaportates it pulls the other water up with it
Adaptions of terrestrial plants to water loss
xerophytes - plants adapted to dry habitats
adaptions include:
- sunken stomata
- stomatal hairs
- thick waxy cuticle
- rolled leaves
- fewer stomata
Haemodialysis Pros and Cons
Pros - requires no training, only 4 days a week, can do non active activities at the same time, flexible treatment times
Cons - limited fluid and diet intake, may feel washed out afterwards, needle sticks, must travel to centre
Haemoglobin advantage
- very compact - each RBC can carry approximately 300 million
- 02 doesnt dissolve well in solution therefore hB affinity to 02 increases the bloods capacity to transport oxygen
- At high altitudes one response is increased production of RBCs and therefore more hB
- ~23% of carbon dioxide combines with haemoglobin to form HHb which buffers the blood to keep pH change minimum
- bohr effect
Alleles
alleles are alternate forms of genes
alleles that mask other alleles are dominant
alleles that are masked are recessive
alleles expressed equally are codominant
Haemoglobin
protein with a quaternary structure = 4 polypeptide chains each with a haem (Fe) group
Each RBC contains ~300 million Hb molecules
May combine with oxygen to form oxyhaemoglobin or with carbon dioxide to form HHb
Induced fit model
model describing enzyme specificity
enzyme and substrate dont have exact complementary shapes but active site changes size to accomodate
Enzyme returns to normal shape after reaction has taken place
Relationship between arteries, veins and capillaries
Blood flow from heart ---> arteries ---> arterioles ---> capillaries ---> venules ---> veins ---> blood flow from heart
Experiment on natural selection
Aim: to model natural selection
Hypothesis: the varient most suited to the selective pressure will become predominant
Risk: Small disc may become a slipping hazard if on the floor. As such pick up any fallen chips immeadiatly
Materials: 20x blue chips 20x pink chips 20x orange 20x green 20x white 1x floral background
Method: 1. have one experimenter randomly arrange all the chips on the background 2. Have the other experimenter act as the selective pressure randomly choosing chips 3. count the surviving chips and have each chip 'reproduce' to have 3 same coloured offspring 4. repeat 1-3 two more times
Homozygous vs heterozygous
Heterosygous means that both alleles are different in genotype
Homozygous means that both alleles are the same in genotype
gene definition
length of DNA that codes for a particular polypeptide
monohybrid inheritance
The inheritance of a single trait is controlled by one pair of genes only
Writing up experiments
aim
hypothesis
independant
dependant
controlled variables
control
risk
materials
method
results
graph
discussion
conclusion