Grundlagen der Biologie ll: Zellbiologie
Folgt der Vorlesung Grundlagen der Biologie ll: Zellbiologie der ETZ Zürich
Folgt der Vorlesung Grundlagen der Biologie ll: Zellbiologie der ETZ Zürich
Set of flashcards Details
| Flashcards | 26 |
|---|---|
| Language | Deutsch |
| Category | Biology |
| Level | University |
| Created / Updated | 20.09.2016 / 06.10.2016 |
| Licencing | Not defined |
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Hierarchy of life
Atoms
Molecules
Macromolecules
Cellular organelles
Cells
Tissues
Organs
Organisms
Social and enviromental entities
What determines the functions and properties of a biological unit?
-Intrinsic properties
-The context
Example: Atom in a protein
The properties of an atome are determined by what type of atom it is (C, H, O,..) -->intrinsic but also by its nighboring atoms (protein folding)
Preperative Centrifugation:
Which cell compartements are gained by centrifugating at low, medium, high and ultra speed?
- At low speed: Pellet contains whole cells, nuclei, cytoskeleton
- At medium speed: Mitochondria, lysosomes, peroxisomes
- High speed: Microsomes, small vesicles
- Ultra speed: Ribosomes, viruses, large macromolecules
Chromatography strategies:
- Ion-Exchange
- Gelflitration
-Affinity (common: HIS-Tag)
Micellar concentration (CMC)
Above a criticel micellar concentration, detergents form micelles
Methods to determine protein structur:
-Crystallography
-NMR
-Electron microscopy
Translocation proteins in mitochondria
TOM/SAM-Complex: Translocation between cytosol and intermembran space
TIM22/TIM23/OXA-Complex: Translocation between intermembran space and matrix space
Organells and properties of signal sequences (location, signal removal, nature of signal)
