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Grundlagen der Biologie ll: Zellbiologie

Folgt der Vorlesung Grundlagen der Biologie ll: Zellbiologie der ETZ Zürich

Folgt der Vorlesung Grundlagen der Biologie ll: Zellbiologie der ETZ Zürich

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Kartei Details

Karten 26
Sprache Deutsch
Kategorie Biologie
Stufe Universität
Erstellt / Aktualisiert 20.09.2016 / 06.10.2016
Lizenzierung Keine Angabe
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Hierarchy of life




Cellular organelles





Social and enviromental entities

What determines the functions and properties of a biological unit?

-Intrinsic properties

-The context

Example: Atom in a protein

The properties of an atome are determined by what type of atom it is (C, H, O,..) -->intrinsic but also by its nighboring atoms (protein folding)

Preperative Centrifugation:

Which cell compartements are gained by centrifugating at low, medium, high and ultra speed?

  1. At low speed: Pellet contains whole cells, nuclei, cytoskeleton
  2. At medium speed: Mitochondria, lysosomes, peroxisomes
  3. High speed: Microsomes, small vesicles
  4. Ultra speed: Ribosomes, viruses, large macromolecules

Chromatography strategies:

- Ion-Exchange

- Gelflitration

-Affinity (common: HIS-Tag)

Micellar concentration (CMC)

Above a criticel micellar concentration, detergents form micelles

Methods to determine protein structur:



-Electron microscopy

Translocation proteins in mitochondria

TOM/SAM-Complex: Translocation between cytosol and intermembran space

TIM22/TIM23/OXA-Complex: Translocation between intermembran space and matrix space

Organells and properties of signal sequences (location, signal removal, nature of signal)