Immunology TEST 1

UMED WROC

177

0.0 (0)

A. K.

Lernen

A. K.

Über diese Lernkartei

This flashcard set covers university-level immunology, focusing on cells, antibodies, antigens, and immune responses. It delves into topics like flow cytometry, lymphocyte activation, and the roles of various immune cells such as NK cells and T cells. The flashcards are designed for medical students and professionals seeking to understand the intricate mechanisms of the immune system, including antigen recognition, immune responses, and diagnostic tests like the NBT reduction assay and flow cytometry. The set provides a comprehensive overview of both specific and non-specific immune responses, making it valuable for those studying medical science or pharmaceutics.

Karten

177

Lernende

Sprache

Englisch

Kategorie

Medizin/Pharmazie

Stufe

Universität

Erstellt / Aktualisiert

28.04.2020 / 20.04.2021

Ähnliche Karteien

Lernkarten

An antibody isotype classification results from differences in:

the position of certain ammoniac's in the Ab heavy chain

the position of certain aminocacids in the Ab light chain

the composition of the variable region of the Ab Fab portion

the composition of the Ab heavy and light chains in the constant region

Phagotest in flow cytometry allows the assessment of

Phagocytes capacity of chemotaxis

Phagocytes capacity of proliferation

Phagocytes capacity of pathogen engulfment

Phagocytes capacity of pathogen intracellular killing

An antigen binding site on an antibody molecule is formed by:

solid regions of heavy chains H and light chains L

variable regions of heavy chains H

light chain variable regions L

hypervariable regions of heavy chains H

hypervariable regions of heavy chains H and light chains L

Mitogens are substances used for an in vitro assessment of

(Midterm Set IV)

Respiratory burst in phagocytes

Lymphocyte proliferation

Cell viability

Phagocyte chemotaxis capacity

An Antigen Immunogenicity- this is:

Ability of binding to a specific soluble Immunoglobuline

Ability of binding to a a Immunoglobuline that constitute the B-cell receptor (BCR)

Ability to induce a specific Immune response

Ability to bind specific free Immunoglobulines and T-cell receptors (TCR)

Ability to bind the TCR exclusivelessly

Superantigens can activate T cells in a particular way. This is

A TCR-MHC class I complex binding

A TCR-BCR complex binding

A TCR-MHC class II-complex binding

A Fas-FasL complex binding

An epitope- this is:

an antigen determinant

Exclusively a protein sequence of an antigen {a fragment of a protein only antigen}

An antigen binding site of an antibody

An antibody receptor

An antibody isotype classification results from differences in

The position of certain amino acids in the Ab heavy chain

The position of certain amino acids in the Ab light chain

The composition of the variable region of the Ab Fab portion

The composition of the Ab heavy and light chains in the constant region

An example of a non-specific activation of immunity in the cancer

immunotherapy, is:

the use of monoclonal antibodies conjugated with radioisotopes

the use of vaccines containing tumor antigen-encoding DNA

vaccines containing professional APCs, transfected with genes encoding the cancer antigens

the use of cytokines, such as IL-2, IFN-gamma

the use of monoclonal antibodies conjugated with cytostatics

A naïve lymphocyte, this is a cell which can subsequently turn into

Effector cell

Macrophage

Mast cell

Dendritic cell

An FAB fragment of an Immunoglobulin:

determines the antibody class

determines the antibody subclass

determines the epitope binding properties

determines the complement activation capacity

determines the effectore cellbinding capacity

T CD4+ lymphocyte recognize foreign antigens usually in:

MHC class I restriction

MHC class II restriction

MHC class I and II restriction

Without MHC molecules

The immunofluorescence in flow cytometry can be describes as

The intensity of the emitted florescence is proportional to the number of XXX

The intensity of the emitted florescence is proportional to the number of antigens that bind fluorochrome-labelled antibodies

The intensity of the emitted florescence is proportional to the number antigens binding a fluorochrome

The intensity of the emitted florescence is proportional to the size and granularity of cells

Antigenic determinant is a

paratope

epitope

idiotype

Immunophenotype typical markers for NK cells are:

CD45, CD3

CD19, CD20

CD56, CD16

CD4, CD8

B-cell receptor (BCR), this is:

a soluble antibody

an antibody anchored in the cell membrane

an opsonin receptor

a receptor for the cytokine

Non-oxidative mechanism of intracellular killing in phagocytes are based on XXX

High amount of reactive oxygen species generation

Enzymes activation, mainly peroxidases and NADPH complex

Enzyme activation, mainly proteolytic

Apoptosis activation in the phagocyte

B-lymphocytes have the following characteristics, WITH THE EXCEPTION:

Are more numerous than B virgin lymphocytes

Their BCR receptors have a higher affinity for antigen than in virulent B cells

Have more molecules involved in APC cell adhesion than virgin B cells

Have fewer MHC class II molecules and co-stimulatory molecules than virgin B cells

They are more easily activated than the virgin B cell

CD classification is referred to

Major cell lines surface markers only

Cell lines, maturation and activation cell surface markers

Activation cell surface markers only

CD3 is a typical marker for:

All leukocytes

All T lymphocytes

All B lymphocytes

Th cells only

CD4+ T cells recognize foreign antigens in the restriction of

molecules

MHC class I

MHC class II

MHC class III

APC

Choose the false statement concerning macrophages:

Participate in tissue remodeling

Are the precursors of monocytes

Have toll-like receptors at the cell membrane

Eliminate pathogens by respiratory burst

[Can secrete immunosuppressive agents

Complement plays multiple functions in the immune response, with the exception:

Facilitation opsonization and phagocytosis

Involved in the removal of antigen-antibody complexes

Involved in the activation of apoptosis by death receptors

Involved in chemotaxis

Cytotoxic activity of Tc and NK cells can be assessed in:

BrdU incorporation to DNA test

Tritiated thymidine incorporation to DNA test

Radioactice chromium 51Cr- release from target cells test

Boyden chamber assay

Defensins are natural antibiotic proteins that act on certain microorganisms.

They are produced in large quantities by:

mast cells

NK cells

B-lymphocytes

TCD4 + lymphocytes

Panetha cells in the small intestine

Depending on the type of antigen with which the organism obstructs, a specific

profile of the immune response is formed. Point to the FIRST sentence

describing the immune response:

Th1 response is of particular importance in combating infections caused by intracellular pathogens and plays a key role in the pathogenesis of atypical diseases?

Th2 expression is of particular importance in combating infections caused by parasitic worms and the pathogenesis of atypical diseases

one of the important cytokines secreted by Th2 and IFNgamma T lymphocytes

lymphocytes polarized to the Th17 response do not work Il-17

Il-10 is produced by Th1 lymphocytes and affects the blocking of the Th2 response

ELISA assay in the diagnosis of autoimmune diseases cannot provide:

quantitative assessment of autoantibodies in the serum of the patient

dynamics of the disease process based on changes in the concentration of autoantibodies

determination of autoantibodies titer

autoreactive lymphocytes detection

Fluorochrome labeled antibody application in flow cytometry is

necessary to asses:

cell size

cell cytokine production

cell autofluorescence

cell granularity

Gating- a procedure in flow cytometry, allows:

Selection of distinct subpopulation of cells within the while tested population

Subjecting a selected cell population for further staining

Correct answer a and b

Stopping the flow of the selected population in a flow cytometer and thus avoidance of its assessment

Gell and Coombs-delayed type-responsive effects are related to:

Activation of eosinophils

Release of pro-inflammatory cytokines from monocytes

Release of prostaglandins and leukotrienes from mast cells and basophils

Activation of B lymphocytes by antigen

Activation of T lymphocytes by antigen

Immune cells may have receptors nonspecifically recognizing the pathogen

molecules. Point the receptor NOT related to this aspect of the immune

response.

Lactin receptors

B-cell receptors

Scavenger receptors

toll like receptors

NOD receptors

Immunoadsorbents are:

Antibodies used for immunophenotyping

Antibodies against immunogically competent cells

antibody coated caries

Immunogenecity, is a feature of an antigen, should be defined as:

capability to specifically bind antibodies

capability to specifically bind BCR

capability to induce specific immune response

capability to specifically bind TCR

capability to specifically bind MHC molecules Immunophenotype typical markers for B lymphocytes

Immunophenotype typical markers for B lymphocytes

CD45, CD3

CD19, CD20

CD56, CD16

CD4, CD8

in humans there are

Two subclasses of IgG and four subclasses of IgA

Two subclasses of IgE and four subclasses of IgA

Two subclasses of IgA and four subclasses of IgG

In the cell Immunophenotyping, the B-cell typical markers are:

CD45, CD3

CD19, CD20

CD56, CD16

CD4, CD8

cd14,CD25

In the CSFE cell proliferation assay, in the following cytometry, each subsequent

cell division results in:

decrease in fluorescence intensity in the young cell generation

increase in fluorescent intensity in the young cell generation

decrease in fluorescence intensity in non proliferating cells

decrease in fluorescence intensity in dead cells

In the flow cytometry, the detected fluorescence intensity is proportional to

the general cell number

The fluorochrome-labeled antibody binding antigen number in a particular cell

The number of fluorochromes binding sites in a cell

The size and granularity of the cell

In the humoral immune response to thymus-dependent antigens,

basically the following players participate: 1/ antibodies, 2/T-cells,

3/ cytokines, 4/ B-cells:

1,2

1,2,3

1,3,4

1,2,3,4

1,4

In the non specific cellular immune response generally participates

T cells, B cells, NK cells, and macrophages

NK cells and macrophages

CD4+ T cells

T cells and B cells

T cells and B cells and NK cells

Lernen

Immunology TEST 1

Lernkarteien erstellen oder kopieren

Lernkarten

Bitte melde dich an

Google

Apple ID

Microsoft

Switch edu-ID

Edulog

Google

Apple ID

Microsoft

Switch edu-ID

Edulog